Cytokines in WBC-reduced apheresis PCs during storage: a comparison of twoWBC-reduction methods

Citation
M. Wadhwa et al., Cytokines in WBC-reduced apheresis PCs during storage: a comparison of twoWBC-reduction methods, TRANSFUSION, 40(9), 2000, pp. 1118-1126
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
TRANSFUSION
ISSN journal
0041-1132 → ACNP
Volume
40
Issue
9
Year of publication
2000
Pages
1118 - 1126
Database
ISI
SICI code
0041-1132(200009)40:9<1118:CIWAPD>2.0.ZU;2-6
Abstract
BACKGROUND: Several studies have suggested that cytokine accumulation durin g storage of platelet concentrates (PCs) may mediate nonhemolytic febrile t ransfusion reactions and that a reduction in WBC numbers prevents the gener ation of cytokines. Despite efforts to minimize WBC contamination in aphere sis PCs, high numbers of WBCs and increased cytokine levels may still occur , depending on the quality of the apheresis device employed. STUDY DESIGN AND METHODS: This study was undertaken to investigate whether PCs collected with WBC-reduction devices (Spectra LRS, COBE;or MCS+ LDP, Ha emonetics) were sufficiently depleted of WBCs to limit cytokine accumulatio n during storage. The study evaluated 1) the levels of cytokines of WBC and platelet origin in two types of apheresis PCs during storage and 2) the ef fects of prestorage filtration on cytokine levels in the Spectra LRS PCs. RESULTS: In the Spectra LRS PCs, low levels of IL-6, IL-8, and monotype che moattractant protein 1 (MCP-1) were detected in Day 1 PCs, and they remaine d consistent during the shelf life. RANTES, platelet factor 4 (PF4), beta-t hromboglobulin (beta-TG), and transforming growth factor (TGF)-beta 1 were also detected in these PCs, and their levels increased significantly on sto rage. Prestorage filtration of Spectra LRS PCs did not further reduce the l evels of IL-6, IL-8, MCP-1, PF4, beta-TG, and TGF-beta 1 in the filtered co mponent. In the MCS+LDP PCs, IL-6 was detected on Day 1, and its level incr eased significantly on storage, whereas the levels in the Spectra PCs remai ned steady. IL-8 levels were lower in MCS+LDP PCs than in Spectra LRS PCs o f the same age. MCP-1 levels were similar in both products on Day 1 and mar ginally increased in stored MCS+ LDP PCs. Substantial amounts of RANTES, PF 4, beta-TG, and TGF-beta 1 occurred in Day 1 MCS+LDP PCs, and, on storage, these levels rose significantly. CONCLUSION: Despite a significant reduction in levels of WBC-derived cytoki nes, platelet-derived cytokines were present in different amounts in the tw o products.