Elimination of both cell-free and cell-associated HIV infectivity in plasma by a filtration/methylene blue photoinactivation system

Citation
H. Abe et al., Elimination of both cell-free and cell-associated HIV infectivity in plasma by a filtration/methylene blue photoinactivation system, TRANSFUSION, 40(9), 2000, pp. 1081-1087
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
TRANSFUSION
ISSN journal
0041-1132 → ACNP
Volume
40
Issue
9
Year of publication
2000
Pages
1081 - 1087
Database
ISI
SICI code
0041-1132(200009)40:9<1081:EOBCAC>2.0.ZU;2-6
Abstract
BACKGROUND: Methylene blue phototreatment effectively inactivates cell-free viruses in plasma while maintaining coagulation activities. However, this treatment is considered to be less effective for cell-associated virus inac tivation. This report describes a new virus elimination system designed to eliminate cell-associated viruses with a cell-removal filter followed by me thylene blue photoinactivation of cell-free viruses in plasma. STUDY DESIGN AND METHODS: Fresh plasma was inoculated with HIV or HIV-infec ted Molt4 cells (Molt4(IIIB)). The plasma was transferred to a bag containi ng methylene blue by passing it through a cell-removal filter and was irrad iated with white fluorescent light. HIV infectivity was detected by indirec t fluorescence assay. in parallel studies, coagulation activities in identi cally treated plasma were measured during 1 year of storage at -80 degrees C. RESULTS: Initial cell-free HIV titer of 10(6.2) TCID50 per 0.1 mt dropped t o 10(-0.3) and <10(-0.5) TCID50 per 0.1 mL after 10 or 20 J per cm(2) radia tion, respectively. Cellular components were not detectable in plasma after filtration. The cell-free state of the plasma was ascertained from the obs ervation that the DNase-resistant beta-globin gene, as a marker of intact W BCs, was not detected in the filtrates by PCR. The infectivity of Molt4(III B) was reduced to below the detection limit after filtration and radiation, and proviral HIV DNA was not detected in the filtrates by PCR. Coagulation activities including factor VIII in the treated plasma were maintained at more than 76 percent compared with the percentage in untreated plasma after 1 year of storage. CONCLUSION: The filtration/methylene blue photoinactivation system eliminat ed both cell-free and cell-associated HIV infectivities from plasma while m aintaining coagulation activities for 1 year at -80 degrees C storage.