Ca. Witz et al., Expression of the alpha 2 beta 1 and alpha 3 beta 1 integrins at the surface of mesothelial cells: a potential attachment site of endometrial cells, FERT STERIL, 74(3), 2000, pp. 579-584
Objective: To localize alpha 2 beta 1 and alpha 3 beta 1 integrins in the c
ell membrane of peritoneal mesothelium in vivo and in vitro.
Design: Descriptive study using confocal and two-photon laser-scanning micr
Setting: University-based laboratory.
Patient(s): Women without endometriosis undergoing surgery for benign condi
Main Outcome Measure(s): Peritoneal biopsies (n = 9) and mesothelial monola
yer cultures (n = 4) were incubated with antibodies to the alpha 2 and alph
a 3 subunits and to the intact alpha 2 beta 1 and alpha 3 beta 1 integrins.
Specimens were examined with laser-scanning microscopy.
Result(s): The alpha 2 and alpha 3 subunits and the intact alpha 2 beta 1 a
nd alpha 3 beta 1 integrins were identified at the base of the mesothelial
cells (i.e., toward the basement membrane). There was also expression of th
e alpha 2 and alpha 3 subunits and the intact alpha 2 beta 1 and alpha 3 be
ta 1 integrins at the cell surface (i.e., toward the peritoneal cavity).
Conclusion(s): The resolution of the confocal and two-photon laser-scanning
microscope enabled localization of integrins in mesothelial cells. The pre
sence of alpha 2 beta 1 (collagen-laminin receptor) and alpha 3 beta 1 inte
grins (collagen-laminin-fibronectin receptor) at the base of mesothelial ce
lls suggests a role for these molecules in adhesion to the basement membran
e. The presence of these molecules at the cell surface suggests a potential
locus for cell adhesion in such processes as endometriosis and cancer meta
stasis. (Fertil Steril(R) 2000;74: 579-84. (C) 2000 by American Society for