Expression of the alpha 2 beta 1 and alpha 3 beta 1 integrins at the surface of mesothelial cells: a potential attachment site of endometrial cells

Citation
Ca. Witz et al., Expression of the alpha 2 beta 1 and alpha 3 beta 1 integrins at the surface of mesothelial cells: a potential attachment site of endometrial cells, FERT STERIL, 74(3), 2000, pp. 579-584
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Reproductive Medicine","da verificare
Journal title
FERTILITY AND STERILITY
ISSN journal
0015-0282 → ACNP
Volume
74
Issue
3
Year of publication
2000
Pages
579 - 584
Database
ISI
SICI code
0015-0282(200009)74:3<579:EOTA2B>2.0.ZU;2-8
Abstract
Objective: To localize alpha 2 beta 1 and alpha 3 beta 1 integrins in the c ell membrane of peritoneal mesothelium in vivo and in vitro. Design: Descriptive study using confocal and two-photon laser-scanning micr oscopy. Setting: University-based laboratory. Patient(s): Women without endometriosis undergoing surgery for benign condi tions. Intervention(s): None. Main Outcome Measure(s): Peritoneal biopsies (n = 9) and mesothelial monola yer cultures (n = 4) were incubated with antibodies to the alpha 2 and alph a 3 subunits and to the intact alpha 2 beta 1 and alpha 3 beta 1 integrins. Specimens were examined with laser-scanning microscopy. Result(s): The alpha 2 and alpha 3 subunits and the intact alpha 2 beta 1 a nd alpha 3 beta 1 integrins were identified at the base of the mesothelial cells (i.e., toward the basement membrane). There was also expression of th e alpha 2 and alpha 3 subunits and the intact alpha 2 beta 1 and alpha 3 be ta 1 integrins at the cell surface (i.e., toward the peritoneal cavity). Conclusion(s): The resolution of the confocal and two-photon laser-scanning microscope enabled localization of integrins in mesothelial cells. The pre sence of alpha 2 beta 1 (collagen-laminin receptor) and alpha 3 beta 1 inte grins (collagen-laminin-fibronectin receptor) at the base of mesothelial ce lls suggests a role for these molecules in adhesion to the basement membran e. The presence of these molecules at the cell surface suggests a potential locus for cell adhesion in such processes as endometriosis and cancer meta stasis. (Fertil Steril(R) 2000;74: 579-84. (C) 2000 by American Society for Reproductive Medicine.).