An improved genetic linkage map of rat chromosome 20

Citation
T. Masuyama et al., An improved genetic linkage map of rat chromosome 20, COMPAR MED, 50(4), 2000, pp. 369-373
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Animal Sciences","Animal & Plant Sciences
Journal title
COMPARATIVE MEDICINE
ISSN journal
1532-0820 → ACNP
Volume
50
Issue
4
Year of publication
2000
Pages
369 - 373
Database
ISI
SICI code
1532-0820(200008)50:4<369:AIGLMO>2.0.ZU;2-S
Abstract
Background and Purpose: Rat chromosome 20 is one of special interest becaus e it contains some diabetogenic genes, such as a major histocompatibility c omplex (MHC)-linked genetic components and quantitative trait loci. We stud ied rat chromosome 20, using the backcross progeny between BB/Wor and PVG.R 23 rats, and confirmed the genetic linkage map by use of another backcross panel. Methods: Backcross panels were done between BB/Wor and PVG.R23 rats, and BN and KZC rats. Length variations of simple sequence length polymorphism mar kers were analyzed by use of polymerase chain reaction (PCR) analysis. Alle les of RT1-Bb and RT1-Db were analyzed by use of the PCR-restriction fragme nt length polymorphism method. Genetic maps of rat chromosome 20 were const ructed, using the Map Manager computer program. Results: Fifty-two loci were mapped on rat chromosome 20, Genetic length wa s 57.9 cM, with average spanning of 1.11 cM between markers. The positions of RT1-N1, Tnf, and RT1-Bb into the MHC region were separated and confirmed by results of two backcross panels in our linkage studies. Conclusions: The genetic linkage map of rat chromosome 20 was improved, and was a useful tool for genetic analysis of a diabetogenic gene(s) and for p roducing MHC congenic strains.