Effectiveness of four new pyrazole-pyrimidines on phytopathogens: Ultrastructural evidences on Pythium ultimum

Citation
D. Mares et al., Effectiveness of four new pyrazole-pyrimidines on phytopathogens: Ultrastructural evidences on Pythium ultimum, J PHYTOPATH, 148(7-8), 2000, pp. 395-403
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT
ISSN journal
0931-1785 → ACNP
Volume
148
Issue
7-8
Year of publication
2000
Pages
395 - 403
Database
ISI
SICI code
0931-1785(200008)148:7-8<395:EOFNPO>2.0.ZU;2-O
Abstract
Four newly synthesized molecules derived from pyrazole-pyrimidine were assa yed on Botrytis cinerea Micheli, Fusarium moniliforme Sheld and Pythium ult imuun Trow. All proved effective in inhibiting the growth of the phytopatho gens at all of the test concentrations (10, 20, 50, 100 mu g/ml). The most effective compound was 1-(3)nitrophenyl-6-trifluoromethylpyrazolo[3,4-d]pyr imidine-4(5H)-thione (CF(3)3). Ultrastructural studies on P. ultimum treate d with CF(3)3 revealed alterations in the normal hyphal shape and, at high concentration, plasmolysis and damage to the wall texture was observed. At 20 mu g/ml different vesicles were seen in the cytoplasm: some appeared qui te dense, and specific cytochemical reactions indicated that they were most likely peroxysomes; other vesicles seem to be vacuoles of varying content. In some cases there was disintegration of the nuclear envelope. The effect s on membrane lipids and interference in protein synthesis are hypothesized as possible mechanism of action of the molecule.