Antitumor effects of (1 -> 3)-beta-D-glucan and (1 -> 6)-beta-D-glucan purified from newly cultivated mushroom, Hatakeshimeji (Lyophyllum decastes Sing.)

Citation
Y. Ukawa et al., Antitumor effects of (1 -> 3)-beta-D-glucan and (1 -> 6)-beta-D-glucan purified from newly cultivated mushroom, Hatakeshimeji (Lyophyllum decastes Sing.), J BIOSCI BI, 90(1), 2000, pp. 98-104
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
90
Issue
1
Year of publication
2000
Pages
98 - 104
Database
ISI
SICI code
1389-1723(200007)90:1<98:AEO(-3>2.0.ZU;2-Z
Abstract
Eleven polysaccharides were isolated from a hot-water extract of fruiting b odies of Lyophyllum decastes Sing. by ion-exchange chromatography and gel p ermeation chromatography. Three polysaccharides (IV-I, IV-2, and IV-3) comp osed mainly of glucose showed marked antitumor activities against Sarcoma 1 80 and their average molecular weights were 305 kDa, 130 kDa and 14 kDa, re spectively. From methylation analyses and C-13-NMR spectra, it was suggeste d that IV-I was a (1-->3)-beta-D-glucan-type polysaccharide, IV-3 was a (1- ->6)-beta-D-glucan-type polysaccharide, and IV-2 was a (1-->3, 1-->6)-beta- D-glucan-type polysaccharide or a mixture of both polysaccharides. Increase s in the number of peritoneal macrophages and third component of complement (C3)-positive fluorescent cells in mice treated with IV-1 suggested that t he inhibitory effect on tumor growth is due to immunological host-mediated mechanisms.