Identification of a novel gene, GASC1, within an amplicon at 9p23-24 frequently detected in esophageal cancer cell lines

Citation
Zq. Yang et al., Identification of a novel gene, GASC1, within an amplicon at 9p23-24 frequently detected in esophageal cancer cell lines, CANCER RES, 60(17), 2000, pp. 4735-4739
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER RESEARCH
ISSN journal
0008-5472 → ACNP
Volume
60
Issue
17
Year of publication
2000
Pages
4735 - 4739
Database
ISI
SICI code
0008-5472(20000901)60:17<4735:IOANGG>2.0.ZU;2-T
Abstract
In a recent study, we identified frequent amplification of DNA copy number at chromosome 9p23-24 in cell lines derived from esophageal squamous cell c arcinomas (ESCs), using comparative genomic hybridization, Because amplifie d regions often harbor oncogenes and/or other tumor-associated genes, and b ecause 9p23-24 amplification had been reported in various other types of ca ncers, we used fluorescence in situ hybridization and Southern blot analysi s to map the 9p23-24 amplicon, We then screened target genes/transcripts pr esent within this amplicon by Northern blotting. With this strategy, we suc cessfully cloned a novel gene, designated gene amplified in squamous cell c arcinoma 1 (GASC1), that was amplified and overexpressed in several ESC cel l lines. The deduced amino acid sequence of GASC1 contains two PHD-finger m otifs and a PX domain. PHD-finger motifs are found in nuclear proteins that participate in chromatin-mediated transcriptional regulation and are prese nt in a number of proto-oncogenes, Our findings suggest that overexpressed GASC1 may play an important role in the development and/or progression of v arious types of cancer including ESC.