Quantitative detection of eight phthalate metabolites in human urine usingHPLC-APCI-MS/MS

Citation
Bc. Blount et al., Quantitative detection of eight phthalate metabolites in human urine usingHPLC-APCI-MS/MS, ANALYT CHEM, 72(17), 2000, pp. 4127-4134
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
0003-2700 → ACNP
Volume
72
Issue
17
Year of publication
2000
Pages
4127 - 4134
Database
ISI
SICI code
0003-2700(20000901)72:17<4127:QDOEPM>2.0.ZU;2-C
Abstract
Because of the ubiquity of phthalates and their potential role in increasin g risk for cancer and reproductive dysfunction, the need for human exposure assessment studies is urgent. In response to this need, we developed a hig h-throughput, robust, sensitive, accurate, and precise assay for simultaneo us measurement of trace levels of eight phthalate metabolites in human urin e by HPLC-MS/MS. Human urine samples were processed using enzymatic deconju gation of the glucuronides followed by solid-phase ex-traction. The eluate was concentrated, and the phthalate metabolites were chromatographically re solved by reversed-phase HPLC, detected by APCI-tandem mass spectrometry, a nd quantified by isotope dilution. This selective analytical method permits rapid detection (7.7 min total run time) of eight urinary metabolites of t he most commonly used phthalates with detection limits in the low nanagram per milliliter range. Assay precision Was improved by incorporating C-13(4) -labeled internal standards for each of the eight analytes, as well as a co njugated internal standard to monitor deconjugation efficiency. This select ive, sensitive, and rapid method will help elucidate potential associations (if any) between human exposure to phthalates and adverse health effects.