Suppression of azoxymethane-induced colon carcinogenesis in male F344 ratsby mandarin juices rich in beta-cryptoxanthin and hesperidin

Citation
T. Tanaka et al., Suppression of azoxymethane-induced colon carcinogenesis in male F344 ratsby mandarin juices rich in beta-cryptoxanthin and hesperidin, INT J CANC, 88(1), 2000, pp. 146-150
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF CANCER
ISSN journal
0020-7136 → ACNP
Volume
88
Issue
1
Year of publication
2000
Pages
146 - 150
Database
ISI
SICI code
0020-7136(20001001)88:1<146:SOACCI>2.0.ZU;2-F
Abstract
We have reported protective effects of dietary administration of a powder " CHRP" containing high amounts of beta-cryptoxanthin and hesperidin prepared from a Satsuma mandarin (Cirrus unshiu Marc.) juice on azoxymethane (AOM)- induced rat aberrant crypt foci through suppression of crypt: cell prolifer ation and/or induction of detoxifying enzymes. In the present study, we inv estigated the modifying effects of a commercial Satsuma mandarin (Citrus un shiu Marc.) juice (MJ) and those of MJ2 and MJ5, which were prepared from M J and are richer in beta-cryptoxanthin and hesperidin than MJ, on the occur rence of colonic tumors induced by AOM in male F344 rats. Rats were given 2 weekly s,c. injections of AOM (20 mg/kg body weight) to induce colonic neo plasms. They also received MJ, MJ2, or MJ5 as a drinking water at night for 36 weeks, starting 1 week after the last dosing of AOM. AOM exposure produ ced colonic adenocarcinoma with an incidence of 69% and a multiplicity of 0 .76 +/- 0.57/rat at week 38, MJ, MJ2, and MJ5 administration significantly reduced the frequency of colonic carcinoma [M]: 35% (49% reduction), p < 0. 02; MJ2: 20% (64% reduction), p = 0.0028; and MJ5: 15% (78% reduction), p < 0.00021] and multiplicity [MJ: 0.40 +/- 0.58 (47% reduction), p < 0.05; MJ 2: 0.25 +/- 0.43 (67% reduction), p < 0.005; and MJ5: 0.15 +/- 0.36 (80% re duction), p < 0.0011, Also, the numbers of cancer cells positive for prolif erative cell nuclear antigen (PCNA) and cyclin D1 in colonic tumors were lo wered by these treatments. In addition, treatment with MJ, MJ2, or MJ5 sign ificantly increased apoptotic index in colonic adenocarcinoma. These findin gs might suggest effective chemopreventive ability of MJs, especially MJ5, in colon tumorigenesis. (C) 2000 Wiley-Liss, Inc.