Pathologic findings and association of Mycobacterium bovis infection with the bovine NRAMP1 gene in cattle from herds with naturally occurring tuberculosis

Citation
R. Barthel et al., Pathologic findings and association of Mycobacterium bovis infection with the bovine NRAMP1 gene in cattle from herds with naturally occurring tuberculosis, AM J VET RE, 61(9), 2000, pp. 1140-1144
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
AMERICAN JOURNAL OF VETERINARY RESEARCH
ISSN journal
0002-9645 → ACNP
Volume
61
Issue
9
Year of publication
2000
Pages
1140 - 1144
Database
ISI
SICI code
0002-9645(200009)61:9<1140:PFAAOM>2.0.ZU;2-7
Abstract
Objective-To determine necropsy and Mycobacterium bovis culture results in cattle from herds with tuberculosis, the role of the bovine NRAMP1 gene rn resistance and susceptibility to infection with M bovis, and the associatio n between magnitude of the tuberculous lesions and various types of M bovis isolates. Animals-61 cattle from herds with tuberculosis in Texas and Mexico. Procedure-61 cattle were evaluated by necropsy; 59 had positive and 2 had n egative caudal fold tuberculin intradermal lest (CFT) results. Thirty-three cattle with positive CFT results were genotyped to evaluate polymorphism o f the 3' untranslated region of the bovine NRAMP1 gene, using single-strand ed conformational analysis, 9 were resistant to M bovis with no tuberculous lesions and negative M bovis culture results, and 24 were susceptible with tuberculous lesions and positive M bovis culture results. Isolates of M bo vis were analyzed by restriction fragment length polymorphism (RFLP) on the basis of IS6110 sequences and direct-repeat fingerprinting patterns. Results-21 (35.6%; 21/59) cattle with positive CFT results had tuberculous lesions or positive culture results; in addition, 1 of 2 cattle with negati ve CFT results had tuberculous lesions and positive culture results. Tuberc ulous lesions were most common in the thorax (35/63, 55.5%) and lymphoid ti ssues of the head (10/63; 15.9%). Tuberculous lesions varied from 1 to 11/a nimal; 8 of 21 (38.1%) had solitary lesions. Associations were not found be tween resistance or susceptibility to infection with M bovis and polymorphi sm in the NRAMP1 gene or between the magnitude of the lesions and various R FLP types of M bovis isolates. Conclusions and Clinical Relevance-The NRAMP1 gene does not determine resis tance and susceptibility to infection with M bovis in cattle.