Identification of genes involved in mucosal defense and inflammation associated with normal enteric bacteria

Citation
H. Ogawa et al., Identification of genes involved in mucosal defense and inflammation associated with normal enteric bacteria, AM J P-GAST, 279(3), 2000, pp. G492-G499
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
ISSN journal
0193-1857 → ACNP
Volume
279
Issue
3
Year of publication
2000
Pages
G492 - G499
Database
ISI
SICI code
0193-1857(200009)279:3<G492:IOGIIM>2.0.ZU;2-B
Abstract
Normal luminal bacteria and their products play a role in experimental coli tis and inflammatory bowel disease. However, what molecules from what cells are responsible for mounting and maintaining the mucosal defense against l uminal flora is still uncertain. The aim of this study was to identify epit helial gene products involved in mucosal defense and inflammation associate d with ubiquitous enteric bacteria. Germ-free ICR mice were given an oral b acterial suspension prepared from conventional components (bacterial recons titution). Small intestinal and colonic epithelial cells were isolated from bacteria-reconstituted, germfree, and specific pathogen-free mice. Differe ntial gene expression was investigated by differential display, Northern bl ot, and sequence analysis. Bacterial reconstitution resulted in acute but s elf-limited colitis. In epithelial cells, we observed the induction of smal l intestine-specific genes of the cryptdin family and colon-specific expres sion of serum amyloid A1 gene. This novel approach allows the identificatio n of known and novel gene products involved in mucosal defense against lumi nal microorganisms and the associated inflammatory response.