Increased exon 5 expression alters extrasynaptic NMDA receptors in cerebellar neurons

Citation
K. Prybylowski et al., Increased exon 5 expression alters extrasynaptic NMDA receptors in cerebellar neurons, J NEUROCHEM, 75(3), 2000, pp. 1140-1146
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROCHEMISTRY
ISSN journal
0022-3042 → ACNP
Volume
75
Issue
3
Year of publication
2000
Pages
1140 - 1146
Database
ISI
SICI code
0022-3042(200009)75:3<1140:IE5EAE>2.0.ZU;2-6
Abstract
We investigated the ontogenic changes in expression of alternatively splice d forms of NR1 protein that contain the N1 cassette (exon 5) in comparison with the total population of NR1 within the rat cerebellum. The N1 cassette is strongly developmentally regulated in the cerebellum, with >80% of tota l NR1 protein in the adult rat containing the N1 cassette. In contrast, ear ly in development, <20% of NR1 protein contained this cassette. Rat cortice s from the same ages did not show an increase in the percent of NR1 protein expressing the N1 cassette, indicating that the developmental changes in t he cerebellum are tissue-specific. As the N1 cassette is known to determine NMDA receptor properties, including spermine sensitivity and decay kinetic s of glutamate-induced currents, changes in the characteristics of glutamat e-activated currents in granule cells from cerebellar slices were compared at postnatal day 7 versus 14. Glutamate responses exhibited fast deactivati on kinetics and reduced potentiation by spermine at day 14 while maintainin g sensitivity to an NR2B-selective antagonist. These data are consistent wi th the possibility that N1 cassette expression may be a factor in the devel opmental changes in properties of extrasynaptic NMDA receptors in the cereb ellum.