Breeding of high ergosterol-producing yeast strains

Citation
X. He et al., Breeding of high ergosterol-producing yeast strains, J IND MIC B, 25(1), 2000, pp. 39-44
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSN journal
1367-5435 → ACNP
Volume
25
Issue
1
Year of publication
2000
Pages
39 - 44
Database
ISI
SICI code
1367-5435(200007)25:1<39:BOHEYS>2.0.ZU;2-J
Abstract
High ergosterol-producing yeast strains YEH-28 and YEH-56 were constructed by hybridization of two haploids with opposite mating types from different species. The fermentation conditions of hybrid strain YEH-56 were studied. The highest level of ergosterol was obtained in 30 h at 28 degrees C and 20 0 rpm, when 60 ml of culture in 250-ml shake flasks was grown in fermentati on medium that consisted of (per liter): 100 g glucose; 10 g peptone and 10 g yeast extract. The initial pH was adjusted to 6.0, The optimal inoculum volume was 10% (v/v). Under optimal conditions, the yield of ergosterol of YEH-28 strain is 1.96 and 1.56 times that of the parental strains YE39 and YE244, whereas that of YEH-56 is 1.98 and 1.57 times that of the parental s trains YE39 and YE244, respectively. Analysis of genetic stability showed t hat hybrid strains YEH-28 and YEH-56 are stable genetically.