Approaches to functional genomics: potential of matrix-assisted laser desorption ionization-time of flight mass spectrometry combined with separationmethods for the analysis of DNA in biological samples

Citation
Kg. Lichtenwalter et al., Approaches to functional genomics: potential of matrix-assisted laser desorption ionization-time of flight mass spectrometry combined with separationmethods for the analysis of DNA in biological samples, J CHROMAT B, 745(1), 2000, pp. 231-241
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
1387-2273 → ACNP
Volume
745
Issue
1
Year of publication
2000
Pages
231 - 241
Database
ISI
SICI code
1387-2273(20000804)745:1<231:ATFGPO>2.0.ZU;2-I
Abstract
MALDI-TOF MS has potential as a valuable technique in DNA mapping studies a nd may well be complementary to other approaches to DNA analysis such as ge l electrophoresis and sequencing. This study used 2,6-dihydroxyacetophenone (DHAP) mixed with diammonium hydrogen citrate (DAHC) as the matrix. In add ition, recent technical advances such as time lag focussing (TLF) and bette r selection of matrices (such as 3-hydroxypicolinic acid (3 HPA) and picoli nic acid (PA)) extended the range of DNA fragments that can be studied by t his approach. The following samples were investigated: Poly-T mixture (dT 1 5, 19, 20, 25, 74 and 75), plasmid pBR322 derived oligonucleotides (10, 11, 12, 13, 14, 15, 19, 20 and 50 nucleotides long) and DNA fragments of 25, 3 6 and 37 base pairs corresponding to a fragment in the restriction map for the gene corresponding to the hexon protein of Adenovirus 2 and 5, The resu lts were contrasted with similar analyses performed by ion-paired reversed- phase HPLC coupled to on-line electrospray mass spectrometry. (C) 2000 Else vier Science BN. All rights reserved.