The accumulation of heat-shock HSP70 and MXR proteins in the digestive glan
d and gills respectively of the mussel (Mytilus galloprovincialis) has been
examined in individuals collected monthly during 1 year from the maricultu
re areas located in the Ebro Delta (NW Spain). Both proteins were visualise
d and semi-quantified by western blot using commercially available monoclon
al antibodies and subsequent image analysis. Two HSP-related bands of Mr 72
and 78 kDa and two MXR proteins of Mr 230 and 130 kDa were detected and th
eir seasonal variations in expression levels recorded. A significant correl
ation between HSP70 and MXR expression was obtained, the maximum protein ac
cumulation being detected during the summer period. These two parameters we
re also significantly correlated to water temperature at sample sites. Thes
e results suggest either a direct role of temperature in protein expression
or an indirect role driven by food availability and growth. (C) 2000 Elsev
ier Science B.V. All rights reserved.