Seasonal variation of MXR and stress proteins in the common mussel, Mytilus galloprovincialis

Citation
C. Minier et al., Seasonal variation of MXR and stress proteins in the common mussel, Mytilus galloprovincialis, AQUAT TOX, 50(3), 2000, pp. 167-176
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aquatic Sciences
Journal title
AQUATIC TOXICOLOGY
ISSN journal
0166-445X → ACNP
Volume
50
Issue
3
Year of publication
2000
Pages
167 - 176
Database
ISI
SICI code
0166-445X(200009)50:3<167:SVOMAS>2.0.ZU;2-3
Abstract
The accumulation of heat-shock HSP70 and MXR proteins in the digestive glan d and gills respectively of the mussel (Mytilus galloprovincialis) has been examined in individuals collected monthly during 1 year from the maricultu re areas located in the Ebro Delta (NW Spain). Both proteins were visualise d and semi-quantified by western blot using commercially available monoclon al antibodies and subsequent image analysis. Two HSP-related bands of Mr 72 and 78 kDa and two MXR proteins of Mr 230 and 130 kDa were detected and th eir seasonal variations in expression levels recorded. A significant correl ation between HSP70 and MXR expression was obtained, the maximum protein ac cumulation being detected during the summer period. These two parameters we re also significantly correlated to water temperature at sample sites. Thes e results suggest either a direct role of temperature in protein expression or an indirect role driven by food availability and growth. (C) 2000 Elsev ier Science B.V. All rights reserved.