Bioaugmentation with the clc-element carrying Pseudomonas putida BN210 in a membrane separation bioreactor

Ghyoot, W Springael, D Dong, Q Van Roy, S Nuyts, G Diels, L

W. Ghyoot et al., Bioaugmentation with the clc-element carrying Pseudomonas putida BN210 in a membrane separation bioreactor, WATER SCI T, 41(10-11), 2000, pp. 279-286

12

INGLESE

Article

Environment/Ecology

WATER SCIENCE AND TECHNOLOGY

0273-1223 → ACNP

41

10-11

2000

279 - 286

ISI

0273-1223(2000)41:10-11<279:BWTCCP>2.0.ZU;2-F

For the removal of toxic and recalcitrant organic substances from wastewate r, bioaugmentation with bacteria harbouring specific degradation pathways i n activated sludge could constitute a relevant process. In this study, we e xamined the behaviour of a 3-chlorobenzoate (3CBA) degrading Pseudomonas pu tida BN210 carrying the self-transferable c/c-element containing the c/c-ge nes for 3CBA metabolism in a conventional activated sludge system (CAS) and a membrane separation bioreactor (MBR). It was shown that bioaugmentation in the MBR resulted in an increased resistance towards shock loading of 3CB A in terms of improved COD removal. Respirometry showed that the MBR sludge was less destabilized by 3CBA shock loadings than the CAS sludge. Molecula r techniques including PCR-DGGE to follow strain BN210 and PCR using specif ic primer sets to follow the c/c-element showed that strain BN210 was rapid ly lost in both reactors or seems only to survive in low cell numbers. Othe r 3CBA degrading populations were observed in both CAS and MBR reactors.