Concordance of human papillomavirus in the cervix and urine among inner city adolescents

Citation
Dl. Jacobson et al., Concordance of human papillomavirus in the cervix and urine among inner city adolescents, PEDIAT INF, 19(8), 2000, pp. 722-728
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease
Journal title
PEDIATRIC INFECTIOUS DISEASE JOURNAL
ISSN journal
0891-3668 → ACNP
Volume
19
Issue
8
Year of publication
2000
Pages
722 - 728
Database
ISI
SICI code
0891-3668(200008)19:8<722:COHPIT>2.0.ZU;2-G
Abstract
Objectives. To evaluate concordance for human papillomaviruses (HPVs) betwe en cervix and urine in sexually active adolescents. Methods. Cervical swabs and urine were collected from 80 adolescents in Bal timore, MD. Specimens were tested for 34 HPVs by PCR and for cancer-associa ted HPVs by Hybrid Capture (HC II) Probe B. Cervical vs. urine prevalence w as evaluated by logistic regression with general estimating equations. Risk factors for prevalence and viral burden were evaluated by Fisher's exact a nd Kruskal-Wallis tests, respectively. Results. HPV prevalence by PCR, for any HPV, was very high in the cervix (9 0.0%) and somewhat lower in urine (75.0%) (odds ratio, 1.07; 95% confidence interval 1.07 to 1.34). Only one adolescent was HPV-positive in urine alon e. Among HPV-PCR positives at either or both sites, concordance was 82% for presence of any HPV and 40% for specific HPV types. Multiple infections we re common at both sites. On an average, HC II viral burden (relative light unit ratio) was 9-fold higher in cervix than in urine (median, 47.3 vs. 4.9 ; P = 0.005) but correlated poorly between the two sites of the same indivi dual (r = 0.14). Compared with normal adolescents, those with squamous intr aepithelial lesions had a much higher prevalence of HPV by HC II in cervix (100% vs. 28.6, P < 0.0001) as well as in urine (86.7% vs. 35.4%, P = 0.002 ) and a significantly higher viral burden in the cervix (median, 141.8 vs. 7.3, P = 0.0045) but not in urine (median, 22.7 vs. 4.38; P = 0.13). Conclusion. There was a very high prevalence of HPV in cervix and urine of sexually active adolescents. Testing urine for HPV may be useful in epidemi ologic investigations and in monitoring of infected women.