Generation of dendritic cells from human chronic myelomonocytic leukemia cells in fetal calf serum-free medium

Citation
L. Oehler et al., Generation of dendritic cells from human chronic myelomonocytic leukemia cells in fetal calf serum-free medium, LEUK LYMPH, 38(5-6), 2000, pp. 577
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology,"Onconogenesis & Cancer Research
Journal title
LEUKEMIA & LYMPHOMA
ISSN journal
1042-8194 → ACNP
Volume
38
Issue
5-6
Year of publication
2000
Database
ISI
SICI code
1042-8194(200008)38:5-6<577:GODCFH>2.0.ZU;2-7
Abstract
It is generally believed that the immune system plays a role not only in th e acquisition of malignant diseases but also in the rejection of microscopi c as well as established tumor cells. Failure of the immune system to elimi nate tumor cells may be, among other factors, due to an insufficient presen tation of tumor antigens. Dendritic cells (DCs), as professional antigen-pr esenting cells., therefore, may be therapeutically used to initiate or enha nce immune responses in patients; with malignancies. In this study we demon strate that peripheral blood cells of patients with chronic myelomonocytic leukemia (CMML) can be induced to acquire DC characteristics. Upon culture with granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interle ukin-4 (IL-4) plus tumor necrosis factor alpha (TNF alpha), CMML cells deve lop DC morphology and acquire the phenotypic characteristics of DCs, When a CD14(+) cell population is used for DC generation, a homogeneous different iation towards the DC lineage occurs similar to that, observed in normal pe ripheral blood monocytes, CMML-derived DCs are potent stimulators of the al logeneic mixed lymphocyte reaction (MLR) when compared with uncultivated ce lls, The demonstration of a deletion of the long arm of chromosome 7, del(7 )(q22), in 86% of highly enriched CD1a(+) cells by fluorescence in situ hyb ridization (FISH) indicates the leukemic origin of generated DCs. In additi on, we present data that generation of CMML-derived DCs is also possible un der fetal calf serum-free conditions for a potential clinical use:. These D Cs may be used as a cellular vaccine to induce anti-tumor immunity in patie nts with CMML.