Propofol improves recovery from paraquat acute toxicity in vitro and in vivo

Citation
J. Ariyama et al., Propofol improves recovery from paraquat acute toxicity in vitro and in vivo, INTEN CAR M, 26(7), 2000, pp. 981-987
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aneshtesia & Intensive Care
Journal title
INTENSIVE CARE MEDICINE
ISSN journal
0342-4642 → ACNP
Volume
26
Issue
7
Year of publication
2000
Pages
981 - 987
Database
ISI
SICI code
0342-4642(200007)26:7<981:PIRFPA>2.0.ZU;2-S
Abstract
Objective:To investigate whether the antioxidative sedatives propofol and t hiopental can improve recovery from acute paraquat toxicity in A549 cells a nd in mice. Design: Prospective, controlled, dose-response, in vitro study and prospective, controlled animal study. Setting: A university animal rese arch laboratory. Subjects: Established human lung cultured cells and male S PF ICR mice. Interventions: Paraquat-treated (0.2 mM) A549 cells were incub ated either with the antioxidative sedatives propofol (0-0.56 mM) or thiope ntal (0-2.0 mM), or the non-antioxidative sedatives diazepam (0-3.0 mM), mi dazolam (0-3.0 mM) and ketamine (0-9.0 mM), as well as the antioxidative dr ugs, trolox (0-2.0 mM), alpha-tocopherol (0-4.4 mM), antioxidative-processe d food (AOB; 0-1.0 mg/ml), superoxide dismutase (SOD; 0 and 3,000 U/ml) and ulinastatin (0 and 50,000 U/ml), for 48 h. Paraquat treated mice received i. v. injections of 10 mg/kg propofol, 5 mg/kg thiopental, 4.0 mg/kg trolox , 100 mg/kg alpha-tocopherol, 10 mg/kg AOB or 5,000 U/kg SOD, b. i. d. for 4 days (n = 10 each). Measurements and results: Post-administered propofol and thiopental, as well as the antioxidative drugs, trolox, alpha-tocophero l and AOB, improved A549 cell survival in vitro. The non-antioxidative seda tives SOD and ulinastatin were not protective. An i. p. injection of 50 mg/ kg of paraquat resulted in a survival rate of 40 % in mice at day 6. Propof ol, trolox, alpha- tocopherol and AOB significantly lowered the mortality r ate (80 % survival), while thiopental did not. Conclusion: Post i. v. injec tion of propofol is protective against paraquat-induced damage. Propofol ca n be given during mechanical ventilatory support after paraquat poisoning.