Isolation of a novel gene on 8p21.3-22 whose expression is reduced significantly in human colorectal cancers with liver metastasis

Citation
T. Oyama et al., Isolation of a novel gene on 8p21.3-22 whose expression is reduced significantly in human colorectal cancers with liver metastasis, GENE CHROM, 29(1), 2000, pp. 9-15
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
GENES CHROMOSOMES & CANCER
ISSN journal
1045-2257 → ACNP
Volume
29
Issue
1
Year of publication
2000
Pages
9 - 15
Database
ISI
SICI code
1045-2257(200009)29:1<9:IOANGO>2.0.ZU;2-0
Abstract
Metastasis, a major factor contributing to poor prognosis of cancer patient s, is caused by a complex series of events that involve many genes. To inve stigate this process, we analyzed by differential display three cell lines that had been established from a murine colon adenocarcinoma (colon 26), NL 4, NL17, and NL22, each of which possessed a different potential for metast asis in mice. We report here the identification of a novel gene, ream (redu ced expression associated with metastasis), which showed significantly lowe r expression in NL17 and NL22 with a high potential for metastasis than in NL4 without a metastatic potential. The human counterpart of murine ream ex pressed two sizes of transcript, 4.4 kb and 1.8 kb, both encoding the same 367-amino acid peptide, which appeared to contain four membrane-spanning re gions. The cDNA showed no significant homology to any known genes in the pu blic database. Human REAM was found to lie within an 800-kb segment of 8p21 .3-22, where we had previously identified a commonly deleted region in colo rectal and hepatocellular carcinomas. Its expression was reduced in more th an half of the human colorectal cancers we examined, particularly in advanc ed stages with liver metastasis. Furthermore, we identified somatic mutatio ns of this gene in a colorectal cancer, a hepatocellular carcinoma, and a n onsmall lung cancer among I I I human tumors of various stages examined. Ge nes Chromosomes Cancer 29:9-15, 2000. (C) 2000 Wiley-Liss, Inc.