Expression and cellular distribution of perchloric acid-soluble protein isdependent on the cell-proliferating states of NRK-52E cells

Citation
H. Kanouchi et al., Expression and cellular distribution of perchloric acid-soluble protein isdependent on the cell-proliferating states of NRK-52E cells, CELL MOL L, 57(7), 2000, pp. 1103-1108
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
CELLULAR AND MOLECULAR LIFE SCIENCES
ISSN journal
1420-682X → ACNP
Volume
57
Issue
7
Year of publication
2000
Pages
1103 - 1108
Database
ISI
SICI code
1420-682X(200007)57:7<1103:EACDOP>2.0.ZU;2-S
Abstract
To clarify the biological role of kidney perchloric acid-soluble protein 1 (K-PSP1), its expression and intracellular distribution were examined in no rmal rat kidney epithelial NRK-52E cells. K-PSP1 expression was low during the proliferating phase and high in the stationary phase, and shown to have a negative relationship with the protein-synthesizing activity of the cell s. Immunocytochemical studies revealed that K-PSP1 is predominantly located in the cytosol, especially in endoplasmic reticulum and Golgi apparatus of proliferating cells. In the stationary phase, K-PSP1 was not detected immu nologically even though protein and mRNA expression were high. This disappe arance of reactivity with anti-serum seems to be due to a conformational ch ange in K-PSP1 induced by unknown factors. These results suggest that the r ole of K-PSP1 is to regulate cell proliferation, and this may be related to a previously reported ability to inhibit protein synthesis.