Nuclear magnetic resonance studies of the hydration of proteins and DNA

Authors
Citation
Ps. Belton, Nuclear magnetic resonance studies of the hydration of proteins and DNA, CELL MOL L, 57(7), 2000, pp. 993-998
Citations number
26
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Cell & Developmental Biology
Journal title
CELLULAR AND MOLECULAR LIFE SCIENCES
ISSN journal
1420-682X → ACNP
Volume
57
Issue
7
Year of publication
2000
Pages
993 - 998
Database
ISI
SICI code
1420-682X(200007)57:7<993:NMRSOT>2.0.ZU;2-R
Abstract
The behaviour of water in the presence of proteins and DNA as elucidated by nuclear magnetic resonance is reviewed. The picture that emerges is that i n dilute solution only those water molecules in the interior of the biopoly mers or in clefts have their motions substantially affected. In concentrate d systems the situation is more complicated because many more motions have to be considered, but there is no evidence of special effects due to the bi opolymers being present. The case of nonfreezing water in protein solutions is considered, and it is suggested that this is not evidence for 'bound' w ater but simply due to the effects of the inhibition of protein precipitati on.