The involvement of two specific arginine residues in the action of HM-1 killer toxin was deduced from site-directed mutagenesis

Citation
T. Shirai et al., The involvement of two specific arginine residues in the action of HM-1 killer toxin was deduced from site-directed mutagenesis, BIOL PHAR B, 23(8), 2000, pp. 998-1000
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
0918-6158 → ACNP
Volume
23
Issue
8
Year of publication
2000
Pages
998 - 1000
Database
ISI
SICI code
0918-6158(200008)23:8<998:TIOTSA>2.0.ZU;2-2
Abstract
Each of the four arginine residues in the HM-I killer toxin was replaced by alanine using site-directed mutagenesis, The polymerase chain reaction (PC R)-constructed mutant gene was successfully expressed in HM-1 toxin resista nt Saccharomyces cerevisiae. Among four HM-1 toxin analogues, R82A HM-1 tox in and R86A HM-1 toxin lost killer activity, while R61A HM-1 toxin and R85A HM-1 toxin retained activity These results strongly indicate the importanc e of the arginine residues at positions 82 and 86 which are located in the C-terminal region of the HM-1 toxin for the action of killer activity.