Phospholipase A(2) antagonists inhibit constitutive retrograde membrane traffic to the endoplasmic reticulum

Citation
P. De Figueiredo et al., Phospholipase A(2) antagonists inhibit constitutive retrograde membrane traffic to the endoplasmic reticulum, TRAFFIC, 1(6), 2000, pp. 504-511
Citations number
57
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
TRAFFIC
ISSN journal
1398-9219 → ACNP
Volume
1
Issue
6
Year of publication
2000
Pages
504 - 511
Database
ISI
SICI code
1398-9219(200006)1:6<504:PAAICR>2.0.ZU;2-2
Abstract
Eukaryotic cells contain a variety of cytoplasmic Ca2+-dependent and Ca2+-i ndependent phospholipase A(2)s (PLA(2)s; EC 2.3.1.2.3). However, the physio logical roles for many of these ubiquitously-expressed enzymes is unclear o r not known. Recently, pharmacological studies have suggested a role for Ca 2+-independent PLA(2) (iPLA(2)) enzymes in governing intracellular membrane trafficking events in general and regulating brefeldin A (BFA)-stimulated membrane tubulation and Golgi-to-endoplasmic reticulum (ER) retrograde memb rane trafficking, in particular. Here, we extend these studies to show that membrane-permeant iPLA(2) antagonists potently inhibit the normal, constit utive retrograde membrane trafficking from the trans-Golgi network (TGN), G olgi complex, and the ERGIC-53-positive ER-Golgi-intermediate compartment ( ERGIC), which occurs in the absence of BFA. Taken together, these results s uggest that iPLA(2) enzymes play a general role in regulating, or directly mediating, multiple mammalian membrane trafficking events.