Distinction of gametophytes and tetrasporophytes by microfluorometry in Osmundea pinnatifida (Rhodophyta) from Canary Islands

Citation
R. Schnetter et al., Distinction of gametophytes and tetrasporophytes by microfluorometry in Osmundea pinnatifida (Rhodophyta) from Canary Islands, PHYCOLOGIA, 39(2), 2000, pp. 147-152
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aquatic Sciences
Journal title
PHYCOLOGIA
ISSN journal
0031-8884 → ACNP
Volume
39
Issue
2
Year of publication
2000
Pages
147 - 152
Database
ISI
SICI code
0031-8884(200003)39:2<147:DOGATB>2.0.ZU;2-E
Abstract
In Osmundea pinnatifida gametophytic and tetrasporophytic plants can be dis tinguished on the basis of the different DNA content of nuclei in cortical and outer medullary cells obtained from subapical parts of the thallus. No significant differences exist between the two life cycle phases in the DNA content of nuclei of epidermal cells and medullary cells with 10 or more nu clei. Mixed phase thalli producing spermatangia and tetrasporangia have DNA contents in cells with 2-9 nuclei that correspond to gametophytic plants. In central and basal parts of the thallus, cortical and medullary cells of gametophytic and mixed phase thalli attain nuclear DNA content values simil ar to those in the respective tetrasporophytic cells. 1C nuclei contain abo ut 0.23 pg DNA.