Flow-activated Na+ and K+ current in cardiac microvascular endothelial cells

Citation
F. Moccia et al., Flow-activated Na+ and K+ current in cardiac microvascular endothelial cells, J MOL CEL C, 32(8), 2000, pp. 1589-1593
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cardiovascular & Hematology Research
Journal title
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
ISSN journal
0022-2828 → ACNP
Volume
32
Issue
8
Year of publication
2000
Pages
1589 - 1593
Database
ISI
SICI code
0022-2828(200008)32:8<1589:FNAKCI>2.0.ZU;2-S
Abstract
The patch-clamp technique was used to investigate the electrical response o f cardiac microvascular endothelial cells to fluid stream applied through a microtube. The fluid stream induced a membrane current whose amplitude was dependent on now rate. The I-V relationship of the flow-activated current showed a strong inward rectification and a reversal potential close to +30 mV, which gave a P-Na/P-k = 3.47. The inward and outward components of the current nearly disappeared when extracellular Na+ and internal K+, respecti vely, were replaced by NMDG(+). Finally, the flow-activated current was ful ly blocked by 50 mu M Gd3+ and La3+. These results show that cardiac microv ascular endothelial cells respond to mechanical stimulation associated with flow by increasing their permeability to Na+ and K+ This mechanism is sugg ested to contribute to ionic homeostasis at high heart rates and during pos t-ischaemic reperfusion. (C) 2000 Academic Press.