Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms

Citation
A. Moter et Ub. Gobel, Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms, J MICROB M, 41(2), 2000, pp. 85-112
Citations number
160
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF MICROBIOLOGICAL METHODS
ISSN journal
0167-7012 → ACNP
Volume
41
Issue
2
Year of publication
2000
Pages
85 - 112
Database
ISI
SICI code
0167-7012(200007)41:2<85:FISH(F>2.0.ZU;2-Z
Abstract
As a technique allowing simultaneous visualization, identification, enumera tion and localization of individual microbial cells, fluorescence in situ h ybridization (FISH) is useful for many applications in all fields of microb iology. FISH not only allows the detection of culturable microorganisms, bu t also of yet-to-be cultured (so-called unculturable) organisms, and can th erefore help in understanding complex microbial communities. In this review , methodological aspects, as well as problems and pitfalls of FISH are disc ussed in an examination of past, present and future applications. (C) 2000 Elsevier Science B.V. All rights reserved.