Successful preservation of human skin by vitrification

Citation
T. Fujita et al., Successful preservation of human skin by vitrification, J BURN CARE, 21(4), 2000, pp. 304-309
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Surgery
Journal title
JOURNAL OF BURN CARE & REHABILITATION
ISSN journal
0273-8481 → ACNP
Volume
21
Issue
4
Year of publication
2000
Pages
304 - 309
Database
ISI
SICI code
0273-8481(200007/08)21:4<304:SPOHSB>2.0.ZU;2-D
Abstract
The vitrification technique was applied to the preservation of human skin. This technique was simple, and no expensive equipment was needed. Split-thi ckness human skins from 8 patients were immersed in vitrification solution for 10 minutes at room temperature, immediately plunged into a liquid nitro gen tank, and cryopreserved for 3 weeks. The vitrification solution consist ed of 40% ethylene glycol (vol/vol) and phosphate buffered saline solution that contained 30% Ficoll 70 (vol/vol; Wake Junyaku, Co, Tokyo, Japan) and 0.5 mol/L sucrose. The viability of vitrified and cryopreserved skin was ev aluated with the trypan blue dye exclusion test, the methyl-thiazoldiphenyl -tetrazolium (MTT) colorimetric assay, and a culture test of the keratinocy tes obtained from vitrified skin. The results of the trypan blue dye exclus ion test showed 87.4% of viable cells, and MTT developed an average 0.817 a bsorbance. When vitrified skin was compared with 4 degrees C refrigerated s kins after 3 weeks of storage, the difference of viability was significant both on the trypan blue dye exclusion test (P < .05) and on the MTT assay ( P < .01). However, there was no significant difference in the viability of vitrified skins compared with fresh skin. Furthermore, keratinocytes from v itrified skin grew uneventfully in culture test. We used these vitrified sk in allografts for patients with flame burns and electric burns. These allog rafts took well in both cases and promoted wound healing. We concluded that the vitrification method for skin preservation is simple and reliable, and this method could contribute to skin banking.