Identification and characterization of TMEFF2, a novel survival factor forhippocampal and mesencephalic neurons

Citation
M. Horie et al., Identification and characterization of TMEFF2, a novel survival factor forhippocampal and mesencephalic neurons, GENOMICS, 67(2), 2000, pp. 146-152
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENOMICS
ISSN journal
0888-7543 → ACNP
Volume
67
Issue
2
Year of publication
2000
Pages
146 - 152
Database
ISI
SICI code
0888-7543(20000715)67:2<146:IACOTA>2.0.ZU;2-P
Abstract
We have identified a novel mammalian gene, TMEFF2, that encodes a putative transmembrane protein containing two follistatin-like domains and one epide rmal growth factor (EGF)-like domain. The TMEFF2 gene is predominantly expr essed in the brain. In situ hybridization analysis revealed that TMEFF2 is widely expressed in the brain, including hippocampal cornu ammonis, dentate gyrus, and substantia nigra pars compacta. We evaluated the survival effec t of TMEFF2 using primary cultured neurons from several regions of fetal ra t brain following treatment with a recombinant TMEFF2 protein fragment cons isting of the putative extracellular domain. TMEFF2 increased survival of n eurons from the hippocampus and midbrain, but not from the cerebral cortex, indicating that the survival effects of TMEFF2 are specific to certain cel l types. Recombinant TMEFF2 also promoted survival of mesencephalic dopamin ergic neurons. Together, these findings suggest that TMEFF2 may be a novel survival factor for hippocampal and mesencephalic, but not for cortical, ne urons. (C) 2000 Academic Press.