A new method of determination of diffusion coefficients using capillary zone electrophoresis (peak-height method)

Authors
Jin, WR Chen, HF
Citation
Wr. Jin et Hf. Chen, A new method of determination of diffusion coefficients using capillary zone electrophoresis (peak-height method), CHROMATOGR, 52(1-2), 2000, pp. 17-21
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
0009-5893 → ACNP
Volume
52
Issue
1-2
Year of publication
2000
Pages
17 - 21
Database
ISI
SICI code
0009-5893(200007)52:1-2<17:ANMODO>2.0.ZU;2-3
Abstract
A new method of the determination of the diffusion coefficients, D, called "the peak-height method" has been developed. When the zone broadening of a species is solely caused by axial molecular diffusion, its diffusion coeffi cient can be calculated. The diffusion coefficients of L-cysteine and glutathione in a neutral phosp hate buffer were measured by this method. Comparison between the experiment al diffusion coefficient and the one calculated by the Wilke-Chang equation shows that the method gives good results.