A new method of the determination of the diffusion coefficients, D, called
"the peak-height method" has been developed. When the zone broadening of a
species is solely caused by axial molecular diffusion, its diffusion coeffi
cient can be calculated.
The diffusion coefficients of L-cysteine and glutathione in a neutral phosp
hate buffer were measured by this method. Comparison between the experiment
al diffusion coefficient and the one calculated by the Wilke-Chang equation
shows that the method gives good results.