The high-sensitivity determination of protein concentrations by the enhancement of Rayleigh light scattering of Arsenazo-DBN

Citation
Qf. Li et al., The high-sensitivity determination of protein concentrations by the enhancement of Rayleigh light scattering of Arsenazo-DBN, ANALYST, 125(8), 2000, pp. 1483-1486
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYST
ISSN journal
0003-2654 → ACNP
Volume
125
Issue
8
Year of publication
2000
Pages
1483 - 1486
Database
ISI
SICI code
0003-2654(2000)125:8<1483:THDOPC>2.0.ZU;2-B
Abstract
A new Rayleigh light scattering (RLS) assay of protein is presented in this paper. At the optimum pH 4.10, the weak RLS of Arsenazo-DBN can be greatly enhanced by the addition of proteins due to the interaction between protei n and Arsenazo-DBN. Based on this, the reactions of Arsenazo-DBN and protei ns, including bovine serum albumin, human serum album, gamma-globulin, egg albumin, lysozyme and trypsin, were studied. A new quantitative determinati on method for proteins has been developed. The linear range for human serum albumin, for example, is 0.085-34.62 mu g mL(-1) with a detection limit of 44.8 ng mL(-1). Besides high sensitivity, the method is characterized by g ood reproducibility, rapidity of reaction, good stability, and few interfer ing substances. The determination of the proteins in human serum and urine samples by this method give results very close to those obtained using Coom assie Brilliant Blue G-250 colorimetry, with relative standard deviations o f 0.7-2.5%.