Sense transformation reveals a novel role for class I beta-1,3-glucanase in tobacco seed germination

Citation
G. Leubner-metzger et F. Meins, Sense transformation reveals a novel role for class I beta-1,3-glucanase in tobacco seed germination, PLANT J, 23(2), 2000, pp. 215-221
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
23
Issue
2
Year of publication
2000
Pages
215 - 221
Database
ISI
SICI code
0960-7412(200007)23:2<215:STRANR>2.0.ZU;2-F
Abstract
'Coat-enhanced' seed dormancy of many dicotyledonous species, including tob acco, is released during after-ripening. Rupture of the endosperm, which is the limiting step in tobacco seed germination, is preceded by induction of class I beta-1,3-glucanase (beta GLU I) in the micropylar endosperm where the radicle will penetrate. Treating after-ripened tobacco seeds with absci sic acid (ABA) delays endosperm rupture and inhibits beta GLU I induction. Sense transformation with a chimeric ABA-inducible beta GLU I transgene res ulted in over-expression of beta GLU I in seeds and promoted endosperm rupt ure of mature seeds and of ABA-treated after-ripened seeds. Taken together, these results provide direct evidence that beta GLU I contributes to endos perm rupture. Over-expression of beta GLU I during germination also replace d the effects of after-ripening on endosperm rupture. This suggests that re gulation of beta GLU I by ABA signalling pathways might have a key role in after-ripening.