Expression of proliferating cell nuclear antigen (PCNA) in the adult and developing mouse nervous system

Authors
Citation
H. Ino et T. Chiba, Expression of proliferating cell nuclear antigen (PCNA) in the adult and developing mouse nervous system, MOL BRAIN R, 78(1-2), 2000, pp. 163-174
Citations number
47
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
MOLECULAR BRAIN RESEARCH
ISSN journal
0169-328X → ACNP
Volume
78
Issue
1-2
Year of publication
2000
Pages
163 - 174
Database
ISI
SICI code
0169-328X(20000531)78:1-2<163:EOPCNA>2.0.ZU;2-K
Abstract
Proliferating cell nuclear antigen (PCNA) is essential for the function of DNA polymerases delta and epsilon. Because proliferating cell nuclear antig en is required for DNA replication and repair, PCNA is abundantly expressed in proliferating cells. Interestingly, PCNA mRNA has also been detected in the adult mouse brain by Northern blot analysis. In this study, two monocl onal antibodies against PCNA, PC10 and 19F4, were used for Western blot ana lysis. Monoclonal antibody PC10, but not 19F4, detected a band in the adult mouse brain extract. This PC10-reactive protein in the brain displayed a m ore acidic isoelectric point than PCNA by two-dimensional gel electrophores is. In situ hybridization showed that PCNA mRNA was abundantly expressed in the adult mouse subventricular zone. Additionally, relatively low levels o f PCNA mRNA expression were also found in neurons throughout the central ne rvous system, however, no hybridization was observed in the white matter. I mmunohistochemistry was also performed using 19F4 and PC10, and staining of progenitor cell nuclei in the subventricular zone was observed with both a ntibodies. Whereas 19F4 immunostaining was restricted to progenitor cells, PC10 immunostaining was also found in postmitotic nonproliferating cell nuc lei. In the cortical neuroepithelium of developing mice, the distribution o f PC10 immunoreactivity was wider than that of 19F4 immunoreactivity and PC NA mRNA expression. These results suggest that proliferating cell nuclear a ntigen mRNA is expressed not only in proliferating cells but also in nonpro liferating cells such as neurons. The protein recognized only with PC10 may be a modified, most probably a phosphorylated PCNA. (C) 2000 Published by Elsevier Science B.V.