Comparison of separation behavior of benzodiazepines in packed capillary electrochromatography and open-tubular capillary electrochromatography

Citation
K. Jinno et al., Comparison of separation behavior of benzodiazepines in packed capillary electrochromatography and open-tubular capillary electrochromatography, J CHROMAT A, 887(1-2), 2000, pp. 479-487
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
887
Issue
1-2
Year of publication
2000
Pages
479 - 487
Database
ISI
SICI code
0021-9673(20000728)887:1-2<479:COSBOB>2.0.ZU;2-B
Abstract
Packed column capillary electrochromatography (CEC), open-tubular CEC and m icrocolumn liquid chromatography (LC) using a cholesteryl silica bonded pha se have been studied to compare the retention behavior for benzodiazepines. It has been found that packed column CEC gives better resolution, faster a nalysis time than microcolumn LC for benzodiazepines maintaining similar se lectivity except for some solutes which are charged species under the separ ation conditions. However, open-tubular CEC gave different selectivities to a larger extent for charged benzodiazepines from that which should be prod uced by the chromatographic properties of the cholesteryl silica phase. Cha rged species migration times are mainly influenced by electrophoretic mobil ity rather than the chromatographic interactions. (C) 2000 Elsevier Science B.V. All rights reserved.