Comparison of different packing methods for capillary electrochromatography columns

Citation
S. Roulin et al., Comparison of different packing methods for capillary electrochromatography columns, J CHROMAT A, 887(1-2), 2000, pp. 307-312
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
887
Issue
1-2
Year of publication
2000
Pages
307 - 312
Database
ISI
SICI code
0021-9673(20000728)887:1-2<307:CODPMF>2.0.ZU;2-Z
Abstract
A study was carried out in which 50 mu m I.D. fused-silica capillaries were packed with 3 mu m octadecylsilane bonded silica, from the same batch, by four methods; liquid slurry and carbon dioxide supercritical carrier, each with and without the use of an ultrasonic probe. A neutral test mixture was analysed by capillary column in reversed-phase mode, and the reproducibili ty of the electroosmotic flow and of migration time, column efficiency and retention factors, was determined. Initially results suggested that there w as no significant difference between properties of columns packed by differ ent methods, and a more thorough statistical evaluation confirmed this; dif ferences observed in the column performance were attributed to random varia tions between replicate columns, and not between packing methods. However, the variation was least when applying the ultrasonication during liquid slu rry. (C) 2000 Published by Elsevier Science B.V.