Relationships between concentrations of tumor necrosis factor-alpha and nitric oxide in follicular fluid and oocyte quality

Citation
Ks. Lee et al., Relationships between concentrations of tumor necrosis factor-alpha and nitric oxide in follicular fluid and oocyte quality, J AS REPROD, 17(4), 2000, pp. 222-228
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Reproductive Medicine
Journal title
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
ISSN journal
1058-0468 → ACNP
Volume
17
Issue
4
Year of publication
2000
Pages
222 - 228
Database
ISI
SICI code
1058-0468(200004)17:4<222:RBCOTN>2.0.ZU;2-L
Abstract
Purpose: Our objective was to explain a relationship between concentrations of tumor necrosis factor-alpha. (TNF-alpha) and nitric oxide (NO) in folli cular fluid, oocyte quality, and outcomes of in vitro fertilization-embryo transfer (NF-ET). Methods: The concentrations of TNF-alpha and NO were measured in 115 follic ular fluid samples collected from 43 patients undergoing IVF-ET program, du e to tribal obstruction, some with endometriosis (8 patients) or hydrosalpi nx (5 patients). A correlation of these factors concentrations and the oocy te quality the oocyte maturity, and infertility-associated diseases was ana lyzed. Results: No correlation was found between concentrations of NO and TNF-alph a in follicular fluid. NO concentrations in follicular fluids were signific antly higher in patients with endometriosis (P < 0.001) or hydrosalpinx (P < 0.01) compared to the patients with just tubal obstruction. Follicular NO concentration differences according to oocyte maturity and oocyte quality were not found. In contrast, TNF-alpha concentrations in follicular fluids were significantly higher in poor quality oocytes (P < 0.05) but were not a ssociated with infertility-associated diseases, such as hydrosalphinx or en dometriosis, and the oocyte maturity. No significant differences in follicu lar levels of NO and TNF-alpha as well as NF-ET parameters of pregnant and nonpregnant groups were revealed. Conclusions: There is ito significant correlation between the concentration s of NO and TNF-alpha in follicular fluid. NO levels in follicular fluid ar e altered infertility-associated diseases. However, TNF-alpha levels but no t NO levels influence oocyte quality. These results suggest that the produc tion of NO and TNF-alpha in follicular fluid may be regulated via different pathways and can be tempered with infertility-associated diseases, thereby influencing oocyte quality locally.