Transformation of plant young proembryos by electroporation

Citation
St. Li et al., Transformation of plant young proembryos by electroporation, CHIN SCI B, 45(13), 2000, pp. 1202-1206
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
CHINESE SCIENCE BULLETIN
ISSN journal
1001-6538 → ACNP
Volume
45
Issue
13
Year of publication
2000
Pages
1202 - 1206
Database
ISI
SICI code
1001-6538(200007)45:13<1202:TOPYPB>2.0.ZU;2-9
Abstract
It is first reported that plant young proembryos expressed exogenous report er genes by electroporation. Young proembryos with 8-32 cells and globular proembryos with 250-400 cells could be isolated by enzymatic maceration com bined with microdissection. After electroporation with GUS or GFP genes, th e proembryos were cultured for 1-2 d in KM8p medium. At the field strength of electroporation 500-1 500 V/cm, blue reaction of GUS or green fluorescen ce of GFP could be observed in the proembryos. The highest transient expres sion frequency of young proembryos (2.2%) was obtained at the field strengt h of 750 V/cm, whereas the highest frequency of globular proembryos (5.9%) was obtained at the field strength of 1 250 V/cm. Taking the proportion of transformed cells in the whole cells of proembryos as efficient transformat ion frequency, the efficient transformation frequency of the young proembry os was 7 times that of the globular proembryos.