Sativin - A novel antifungal miraculin-like protein isolated from legumes of the sugar snap Pisum sativum var. macrocarpon

Ye, XY Wang, HX Ng, TB

Xy. Ye et al., Sativin - A novel antifungal miraculin-like protein isolated from legumes of the sugar snap Pisum sativum var. macrocarpon, LIFE SCI, 67(7), 2000, pp. 775-781

12

INGLESE

Article

Biochemistry & Biophysics

LIFE SCIENCES

0024-3205 → ACNP

67

7

2000

775 - 781

ISI

0024-3205(20000707)67:7<775:S-ANAM>2.0.ZU;2-5

An antifungal protein designated sativin was isolated from the legumes of t he sugar snap (also known as honey pea) Pisum sativum var, macrocarpon. The procedure entailed extraction, affinity chromatography on Affi-gel blue ge l and ion exchange chromatography on CM-Sepharose. The protein exhibited a molecular weight of 38 kDa in SDS-polyacrylamide gel electrophoresis. It po ssessed an N-terminal amino acid sequence which showed similarity to those of miraculin (a sweet protein) and pisavin (a ribosome-inactivating protein from Pisum sativum var arvense Poir manifesting similarity to miraculin). Unlike pisavin, however, sativin demonstrated negligible ribonuclease activ ity and inhibited translation in a rabbit reticulocyte lysate system with a very low potency (IC50 =14 mu M). Sativin exerted antifungal activity agai nst Fusarium oxysporum, Coprinus comatus and Pleurotus ostreatus but not ag ainst Rhizoctonia solani. (C) 2000 Elsevier Science Inc. All rights reserve d.