Influence of gastrointestinal hormones on tumor microcirculation of experimental pancreatic cancer in the rat

Citation
J. Schmidt et al., Influence of gastrointestinal hormones on tumor microcirculation of experimental pancreatic cancer in the rat, DIGEST SURG, 17(3), 2000, pp. 250-255
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Surgery
Journal title
DIGESTIVE SURGERY
ISSN journal
0253-4886 → ACNP
Volume
17
Issue
3
Year of publication
2000
Pages
250 - 255
Database
ISI
SICI code
0253-4886(2000)17:3<250:IOGHOT>2.0.ZU;2-S
Abstract
Background/Aims: Gastrointestinal hormones influence the microcirculation i n the normal pancreas. In the present study, we studied the effect of cerul ein and somatostatin on pancreatic cancer microcirculation after orthotopic and nonorthotopic tumor implantation, Methods: In 36 male Lewis rats (150- 180 g) induction of a ductlike pancreatic cancer was achieved by intrapancr eatic or intraperitoneal tumor fragment interposition between two inert tra nsparent polymethyl methacrylate plates. After 4 weeks, intravital microsco py of the tumor microcirculation was performed in a temperature-controlled immersion chamber. The animals received 5 mu g/kg cerulein or 3 mg/kg somat ostatin for 1 h intravenously. The erythrocyte velocity in normal pancreati c capillaries or in tumor vessels was measured, Results: The erythrocyte ve locity in the capillaries of the normal pancreas was 1.01 +/- 0.11 mm/s at baseline and increased to 1.64 +/- 0.09 mm/s after cerulein stimulation (p = 0.007). Pancreatic cancer vessels demonstrated no increase in erythrocyte velocity after orthotopic (baseline 0.95 +/- 0.14 mm/s, after 1 h 0.86 +/- 0.13 mm/s; n.s.) and nonorthotopic tumor implantation (baseline 0.91 +/- 0 .12 mm/s, after 1 h 0.95 +/- 0.14 mm/s; n.s.) after cerulein stimulation. S omatostatin decreased the erythrocyte velocity both in normal pancreas (bas eline 0.87 +/- 0.02 mm/s, after 1 h 0.60 +/- 0.07 mm/s; p = 0.01) and in pa ncreatic cancer (baseline 0.85 +/- 0.20 mm/s, after 1 h 0.63 +/- 0.18 mm/s; p = 0.02) after orthotopic tumor implantation. There was no effect of soma tostatin after nonorthotopic tumor implantation (baseline 0.90 +/- 0.10 mm/ s, after 1 h 0.88 +/- 0.14 mm/s; n.s.). Conclusion: These data suggest that pancreatic cancer microcirculation lacks physiological blood flow control by stimulatory hormones, in contrast to the normal pancreas. Copyright (C) 2000 S. Karger AG, Basel.