Analytical method for the determination of O,O-diethyl phosphate and O,O-diethyl thiophosphate in faecal samples

Authors
Citation
D. Schenke, Analytical method for the determination of O,O-diethyl phosphate and O,O-diethyl thiophosphate in faecal samples, CHEMOSPHERE, 41(9), 2000, pp. 1313-1320
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Environment/Ecology
Journal title
CHEMOSPHERE
ISSN journal
0045-6535 → ACNP
Volume
41
Issue
9
Year of publication
2000
Pages
1313 - 1320
Database
ISI
SICI code
0045-6535(200011)41:9<1313:AMFTDO>2.0.ZU;2-L
Abstract
A residue analytical method was developed for the determination of the dial kylphosphate metabolites of parathion in faecal samples obtained from rabbi ts. The faecal pieces were homogenised in water and highly water-soluble O, O-diethyl phosphate (DEP) and O,O-diethyl thiophosphate (DETP) were subsequ ently alkylated to pentafluorobenzyl esters by a phase transfer reaction. D erivatisation yields depend on the reaction time. The recovery rates were d etermined over the complete procedure using authentic reference standards i n matrix solution. The reference standards allow to observe an effect of th e sample matrix on the area of signals while GC-FPD is used. The recoveries over the concentration range from 0.05 to 5 mu g/g were 47-62% for O,O-die thyl phosphate and 92-106% for O,O-diethyl thiophosphate potassium salt wit h FPD. (C) 2000 Elsevier Science Ltd. All rights reserved.