TRP gamma, a Drosophila TRP-related subunit, forms a regulated cation channel with TRPL

Citation
Xzs. Xu et al., TRP gamma, a Drosophila TRP-related subunit, forms a regulated cation channel with TRPL, NEURON, 26(3), 2000, pp. 647-657
Citations number
54
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEURON
ISSN journal
0896-6273 → ACNP
Volume
26
Issue
3
Year of publication
2000
Pages
647 - 657
Database
ISI
SICI code
0896-6273(200006)26:3<647:TGADTS>2.0.ZU;2-4
Abstract
TRP and TRPL are two light-sensitive cation channel subunits required for t he Drosophila photoresponse; however, our understanding of the identities, subunit composition, and function of the light-responsive channels is incom plete. To explain the residual photoresponse that remains in the trp mutant , a third TRP-related subunit has previously been proposed to function with TRPL. Here, we identify such a subunit, TRP gamma. We show that TRP gamma is highly enriched in photoreceptor cells and preferentially heteromultimer izes with TRPL in vitro and in vivo. The N-terminal domain of TRP gamma dom inantly suppressed the TRPL-dependent photoresponse, indicating that TRP ga mma-TRPL heteromultimers contribute to the photoresponse. While TRPL and TR P gamma homomultimers are constitutively active, we demonstrate that TRPL-T RP gamma heteromultimers form a regulated phospholipase C- (PLC-) stimulate d channel.