Phylogenetic analysis of rhizosphere-associated beta-subclass proteobacterial ammonia oxidizers in a municipal wastewater treatment plant based on rhizoremediation technology

Citation
Dae. Haleem et al., Phylogenetic analysis of rhizosphere-associated beta-subclass proteobacterial ammonia oxidizers in a municipal wastewater treatment plant based on rhizoremediation technology, LETT APPL M, 31(1), 2000, pp. 34-38
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
LETTERS IN APPLIED MICROBIOLOGY
ISSN journal
0266-8254 → ACNP
Volume
31
Issue
1
Year of publication
2000
Pages
34 - 38
Database
ISI
SICI code
0266-8254(200007)31:1<34:PAORBP>2.0.ZU;2-L
Abstract
In wastewater treatment plants based on the rhizosphere zone (rhizoremediat ion technology), ammonia-oxidizing bacteria (AOB) play an important role in the removal of fixed nitrogen. However, the diversity of these bacteria in rhizoremediation wastewater treatment plants is largely unknown. We employ ed direct PCR amplification and cloning of 16S rRNA genes to determine the phylogenetic affiliation of AOB occurring in root and soil samples of a was tewater treatment plant (Merzdorf plant, Brandenburg, Germany). 16S rDNA cl one libraries were screened by hybridization using an oligonucleotide probe specific for AOB of the beta subclass of proteobacteria. Comparative seque nce analysis of all hybridization-positive clones revealed that the majorit y of rDNA sequences was affiliated to members of the genus Nitrosospira and formed a novel subcluster (SM cluster), whereas only three sequences were most closely related to Nitrosomonas species. Affiliation of the novel Nitr osospira-like sequences with those of isolates from soil and rhizosphere su ggests that phylogenetic clusters reflect physiological differences between members of this genus.