Neuraminidase-producing ability of intestinal bacteria isolated from coastal fish

Citation
H. Sugita et al., Neuraminidase-producing ability of intestinal bacteria isolated from coastal fish, LETT APPL M, 31(1), 2000, pp. 10-13
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
LETTERS IN APPLIED MICROBIOLOGY
ISSN journal
0266-8254 → ACNP
Volume
31
Issue
1
Year of publication
2000
Pages
10 - 13
Database
ISI
SICI code
0266-8254(200007)31:1<10:NAOIBI>2.0.ZU;2-7
Abstract
The neuraminidase-producing ability of bacteria from coastal fish intestine s and their environments was determined using a fluorogenic substrate, 2'-( 4-methylumbelliferyl)-alpha-d-N-acetylneuraminic acid. Of 836 isolates exam ined, 758 (90.7%) produced little or no neuraminidase (< 0.0002 U ml(-1)) w hile 78 (9.3%) produced greater than or equal to 0.0002 U neuraminidase ml( -1). Of note, 10% of vibrios from fish intestines could produce neuraminida ses, and 9% did it efficiently. This result suggests that the vibrios capab le of producing neuraminidases are able to colonize and establish in the in testinal tract of coastal fish to some extent. Additionally, as many as 58% of the Vibrio isolates with high abilities were tentatively identified as Vibrio damsela.