Genotypic characterization of Salmonella typhi by amplified fragment length polymorphism fingerprinting provides increased discrimination as comparedto pulsed-field gel electrophoresis and ribotyping

Citation
S. Nair et al., Genotypic characterization of Salmonella typhi by amplified fragment length polymorphism fingerprinting provides increased discrimination as comparedto pulsed-field gel electrophoresis and ribotyping, J MICROB M, 41(1), 2000, pp. 35-43
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF MICROBIOLOGICAL METHODS
ISSN journal
0167-7012 → ACNP
Volume
41
Issue
1
Year of publication
2000
Pages
35 - 43
Database
ISI
SICI code
0167-7012(200006)41:1<35:GCOSTB>2.0.ZU;2-O
Abstract
Amplified fragment length polymorphism (AFLP) is a recently developed, PCR- based high resolution fingerprinting method that is able to generate comple x banding patterns which can be used to delineate intraspecific genetic rel ationships among bacteria. In the present study, AFLP was evaluated for its usefulness in the molecular typing of Salmonella typhi in comparison to ri botyping and pulsed-field gel electrophoresis (PFGE). Six S. typhi isolates from diverse geographic areas (Malaysia, Indonesia, India, Chile, Papua Ne w Guinea and Switzerland) gave unique, heterogeneous profiles when typed by AFLP, a result which was consistent with ribotyping and PFGE analysis. In a further study of selected S. typhi isolates from Papua New Guinea which c aused fatal and non-fatal disease previously shown to be clonally related b y PFGE, AFLP discriminated between these isolates but did not indicate a li nkage between genotype with virulence. We conclude that AFLP (discriminator y index = 0.88) has a higher discriminatory power for strain differentiatio n among S. typhi than ribotyping (DI = 0.63) and PFGE (DI = 0.74). (C) 2000 Elsevier Science B.V. All rights reserved.