Laminin-coated poly(L-lactide) filaments induce robust neurite growth while providing directional orientation

Citation
N. Rangappa et al., Laminin-coated poly(L-lactide) filaments induce robust neurite growth while providing directional orientation, J BIOMED MR, 51(4), 2000, pp. 625-634
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
ISSN journal
0021-9304 → ACNP
Volume
51
Issue
4
Year of publication
2000
Pages
625 - 634
Database
ISI
SICI code
0021-9304(20000915)51:4<625:LPFIRN>2.0.ZU;2-G
Abstract
Cellular channels during development and after peripheral nerve injury are thought to provide guidance cues to growing axons. In tissue culture where these cues are absent, neurites from dorsal root ganglion neurons grow with a radial distribution. To induce directional axonal growth and to enhance the rate of axonal growth after injury, we have designed microfilaments of poly(L-lactide). We demonstrate that dorsal root ganglia grown on these fil aments in vitro extend longitudinally oriented neurites in a manner similar to native peripheral nerves. The extent of neurite growth was significantl y higher on laminin-coated filaments compared with uncoated and poly-L-lysi ne-coated filaments. As high as 5.8 +/- 0.2 mm growth was observed on lamin in-coated filaments compared with 2.0 +/- 0.2 mm on uncoated and 2.2 +/- 0. 3 mm on poly-L-lysine-coated filaments within 8 days. Schwann cells were fo und to grow on all types of filaments. They were, however, absent in the le ading edges of growth on laminin-coated filaments. Photolysis of Schwann ce lls caused a significant reduction in the neurite length on all types of fi laments. Laminin-coated filaments, however, induced significantly longer ne urites compared with uncoated and/or poly-L-lysine-coated filaments even in the absence of Schwann cells. Our results suggest that laminin-coated poly (L-lactide) filaments are suitable for inducing directional and enhanced ax onal growth. Implants designed by arranging these microfilaments into bundl es should aid regenerating axons by providing guidance cues and channels to organize matrix deposition, cell migration, axon growth, and improve funct ional recovery. (C) 2000 John Wiley & Sons, Inc.