Characterization by atomic force microscopy of fused-silica capillaries chemically modified for capillary electrokinetic chromatography

Citation
Pe. Pullen et al., Characterization by atomic force microscopy of fused-silica capillaries chemically modified for capillary electrokinetic chromatography, ANALYT CHEM, 72(13), 2000, pp. 2751-2757
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
0003-2700 → ACNP
Volume
72
Issue
13
Year of publication
2000
Pages
2751 - 2757
Database
ISI
SICI code
0003-2700(20000701)72:13<2751:CBAFMO>2.0.ZU;2-I
Abstract
Fused-silica capillary tubes with 50-mu m bores have been chemically modifi ed to yield odadecyl-derivatized surfaces with increased surface area. Alky lation is achieved in three steps: etching, silane adlayering, and olefin h ydrosilation. Surface roughness, surface area, surface-tip attractive force s, and topographic images of the native, etched, silane-adlayered, and alky lated capillary bores as measured by atomic force microscopy are presented here. Significant roughening occurs during the etching process, but the mos t dramatic change in roughness and surface adhesion forces occurs in the hy drided (silane-adlayered) capillary surface. Topographic smoothing and pass ivation of surface activity are observed for the alkylated capillary surfac e.