Effect of monocular deprivation on NMDAR1 immunostaining in ocular dominance columns of the marmoset Callithrix jacchus

Citation
C. Fonta et al., Effect of monocular deprivation on NMDAR1 immunostaining in ocular dominance columns of the marmoset Callithrix jacchus, VIS NEUROSC, 17(3), 2000, pp. 345-352
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
VISUAL NEUROSCIENCE
ISSN journal
0952-5238 → ACNP
Volume
17
Issue
3
Year of publication
2000
Pages
345 - 352
Database
ISI
SICI code
0952-5238(200005/06)17:3<345:EOMDON>2.0.ZU;2-Z
Abstract
We previously showed that immunoreactivity to N-Methyl-D-aspartate (NMDA) r eceptors in primary visual cortex of Callithrix jacchus is regulated by vis ual activity during the second and third postnatal months (Fonta et al., 19 97). The purpose of the present study was to show that the columnar pattern of high and low NMDAR1 immunoreactivity observed in monocularly deprived a nimals corresponds to ocular dominance columns linked to the nondeprived an d deprived eye, respectively. We compared cortical distribution of NMDAR1 r eceptors and the projection zones of thalamic afferents, revealed by transn euronal transport of tritiated proline. in 2-month-old, either monocularly deprived or control, marmosets. The data show that ocular dominance columns exist in 2-month-old marmosets and that a 2-week monocular deprivation by means of eyelid suture leads to a modification of the thalamo-cortical affe rents organization. Experiments of neuronal tracing and immunohistochemistr y performed on the same animals demonstrated that cortical domains with dec reased NMDAR1 level correspond to the deprived eve columns. These investiga tions, coupled to the previous results, strongly suggest that the NMDA rece ptors, regulated by visual activity, are involved in the refining of ocular dominance columns in the primary visual cortex of juvenile marmoset.