Detection of tau phosphorylated at threonine 231 in cerebrospinal fluid ofAlzheimer's disease patients

Citation
R. Kohnken et al., Detection of tau phosphorylated at threonine 231 in cerebrospinal fluid ofAlzheimer's disease patients, NEUROSCI L, 287(3), 2000, pp. 187-190
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE LETTERS
ISSN journal
0304-3940 → ACNP
Volume
287
Issue
3
Year of publication
2000
Pages
187 - 190
Database
ISI
SICI code
0304-3940(20000630)287:3<187:DOTPAT>2.0.ZU;2-L
Abstract
A new sandwich ELISA is described which shows specificity for tau phosphory lated at threonine 231 and preferentially reacts with Alzheimer's disease ( AD) brain extracts relative to other dementias. This assay was used to anal yze 58 antemortem cerebrospinal fluid samples. Twenty-three of 27 AD sample s (85% sensitivity) yielded signals greater than the cutoff, while only one of 31 non-AD samples (97% specificity) were greater. This indicates that d etection of phosphotau in cerebrospinal fluid with this sandwich ELISA coul d prove useful in the diagnosis of AD. (C) 2000 Elsevier Science Ireland Lt d. All rights reserved.