Vesicular traffic and Golgi apparatus dynamics during mammalian spermatogenesis: Implications for acrosome architecture

Citation
Rd. Moreno et al., Vesicular traffic and Golgi apparatus dynamics during mammalian spermatogenesis: Implications for acrosome architecture, BIOL REPROD, 63(1), 2000, pp. 89-98
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
BIOLOGY OF REPRODUCTION
ISSN journal
0006-3363 → ACNP
Volume
63
Issue
1
Year of publication
2000
Pages
89 - 98
Database
ISI
SICI code
0006-3363(200007)63:1<89:VTAGAD>2.0.ZU;2-0
Abstract
Vesicular membrane trafficking during acrosome biogenesis in bull and rhesu s monkey spermatogenesis differs from the somatic cell paradigm as imaged d ynamically using the Golgi apparatus probes P-COP, giantin, Golgin-97, and Golgin-95/GM130. In particular, sorting and delivery of proteins seemed les s precise during spermatogenesis. In early stages of spermiogenesis, many G olgi resident proteins and specific acrosomal markers were present in the a crosome. Trafficking in both round and elongating spermatids was similar to what has been described for somatic cells, as judged by the kinetics of Go lgi protein incorporation into endoplasmic reticulum-like structures after brefeldin A treatment. These Golgi components were retrieved from the acros ome at later stages of differentiation and were completely devoid of immatu re spermatozoa. Our data suggest that active anterograde and retrograde ves icular transport trafficking pathways, involving both beta-COP- and clathri n-coated vesicles, are involved in retrieving Golgi proteins missorted to t he acrosome and in controlling the growth and shape of this organelle.