NT2 neurons, a classical model for Alzheimer's disease, are highly susceptible to oxidative stress

Citation
E. Tamagno et al., NT2 neurons, a classical model for Alzheimer's disease, are highly susceptible to oxidative stress, NEUROREPORT, 11(9), 2000, pp. 1865-1869
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROREPORT
ISSN journal
0959-4965 → ACNP
Volume
11
Issue
9
Year of publication
2000
Pages
1865 - 1869
Database
ISI
SICI code
0959-4965(20000626)11:9<1865:NNACMF>2.0.ZU;2-H
Abstract
We have tested undifferentiated NT2 cells as well as differentiated NT2 neu rons (NT2N) for vulnerability to oxidative stress, lipid composition and an tioxidant pattern. NT2N, but not NT2 cells, are highly susceptible to oxida tive stress elicited by different classic pro-oxidant stimuli. In particula r, NT2N cells undergo a high level of oxidative decomposition of omega-3 an d omega-6 polyunsaturated fatty acids (PUFA) of membrane phospholipids, as evaluated by monitoring generation of thiobarbituric reactive substances, 4 -hydroxynonenal (HNE) and chromolipid fluorescent adducts. NT2N cells exhib it low levels of natural antioxidants such as glutathione (GSH) and alpha-t ocopherol and of antioxidant enzymatic activities such as Se-dependent GSH peroxidase and catalase. Accordingly, a direct correlation between lipid pe roxidation and irreversible cell damage is suggested by prevention of NT2N cell death by alpha-tocopherol. NeuroReport 11:1865-1869 (C) 2000 Lippincot t Williams & Wilkins.